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. 2015 Jun 3;21(10):1105–1115. doi: 10.1089/ten.tec.2015.0038

FIG. 4.

FIG. 4.

Results of immunofluorescence microscopy performed to characterize cysts formed as a result of hiPSC differentiation on mesh. (A) Bright field (first image of row) and immunofluorescence images of a cyst stained for hCG-β subunit of hCG hormone (third image). Hoechst-labeled nuclei is shown in the second image, and merged image in the last image of the row. (B) Immunofluorescence images of a cyst showing the expression of CDX2 (third image). (C) Comparative immunofluorescence images of an EB stained for CDX2. Scale bar represent 100 μm. EB, embryonic body; hCG, human chorionic gonadotropin.