FIG. 5.

Results of quantitative analysis of hCG hormone secretion by ELISA. Assayed culture media were collected from EB culture samples (marked as “EBs”) and mesh culture samples with cysts (marked as “TLCs”) at day 15 and 21. Culture medium was not changed the day before media collection to allow for adequate accumulation of the hormone. Data are presented as mean±SD of four replicates (n=4) and double asterisks (**) denote statistical significance, p<0.05. hCG hormone was undetectably low for day 15 EBs and therefore is marked as “undetected.” ELISA, enzyme-linked immunosorbent assay; TLCs, trophoblast lineage cells.