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. 2015 Jun 3;21(10):1105–1115. doi: 10.1089/ten.tec.2015.0038

FIG. 7.

FIG. 7.

Results of immunostaining for E-cadherin and vinculin in hiPSC cultured for 5 days on mesh. Immunofluorescence images showing E-cadherin and Nanog expression (A), and vinculin and Nanog expression (B) by hiPSC on a partially filled mesh. Merged images comprised Hoechst staining of cell nuclei. Comparatively, cells in the mesh interior showed visibly higher expression of E-cadherin stain (white arrow heads in A) compared with cells in contact with or in close proximity to the mesh strands. Contrastingly, the expression of vinculin stain was relatively higher in cells in contact with mesh strands (white arrow heads in B) compared with cells in the mesh interior. Scale bars represent 10 μm in (A), 50 μm in the far left image, and 20 μm in the second to last image of (B). Second to last images are close-up views of the boxed region in the far left image of (B). Blue dotted lines in the far left image of (B) indicate the positions of mesh strands.