Table 2.
Nucleotide and Amino Acid Differences among msp4 Sequences (719 bp) from Anaplasma ovis Strains
| Host | Variant | Sample symbola | Country | GenBankb | msp4 nucleotide positions (amino acid positions)c | Reference | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| 230 (77) | 244 (83) | 470 (157) | 476 (159) | 532 (178) | ||||||
| Sheep | Italy 147 | NId | Italy | AY702924 | G (R) | A (S) | C (A) | C | C (L) | de la Fuente et al. (2005) |
| Italy 20 | NId | Italy | AY702923 | * | * | T (V) | * | * | de la Fuente et al. (2005) | |
| GBK1 | B1; B2; K2; K4 | Tunisia | KC432641 | * | * | * | * | * | Belkahia et al. (2014) | |
| GBK2 | B4; K3 | Tunisia | KC432642 | * | * | T (V) | * | * | Belkahia et al. (2014) | |
| GB3 | B3 | Tunisia | KC432643 | T (I) | * | T (V) | * | * | Belkahia et al. (2014) | |
| GK1 | K1 | Tunisia | KC432644 | T (I) | * | T (V) | * | A (I) | Belkahia et al. (2014) | |
| AoGOv1 | Kh1; Kh2 | Tunisia | KM285218 | * | * | T (V) | * | * | Present study | |
| AoGOv2 | Al1 | Tunisia | KM285219 | G (R) | G (G) | T (V) | * | * | Present study | |
| AoGOv3 | Al2 | Tunisia | KM285220 | T (I) | * | T (V) | A | * | Present study | |
| AoGOv4 | Al3 | Tunisia | KM285221 | G (R) | * | T (V) | A | * | Present study | |
| AoGOv5 | Kh3 | Tunisia | KM285222 | * | * | * | * | * | Present study | |
| Goat | AoGGo1 | Al1–Al5; Sj1–Sj5; Jm1–Jm5; Am1–Am5 | Tunisia | KM285217 | * | * | T (V) | * | * | Present study |
B1–B4 and K1–K4 sheep samples were collected from El Alia (Bizerte governorate) and Sbikha (Kairouan governorate), respectively, by Belkahia et al. (2014). Al1–Al3 and Kh1–Kh3 sheep samples were collected from El Alia (Bizerte governorate) and Khetmine (Bizerte governorate), respectively, in the present study. Al1–Al5, Sj1–Sj5, Jm1–Jm5, and Am1–Am5 goat samples were collected from El Alia, Sejnane, Joumine, and Amdoun, respectively.
GenBank accession number of the variant.
Numbers represent the nucleotide position starting at translation initiation codon Adenine. Conserved nucleotide positions with respect to the Italy 147 strain, Sicily (Italy) are indicated with asterisks (de la Fuente et al., 2005).
NI, not indicated.
Amino acid changes are indicated between parentheses with single letter code.
Amino acids: R, arginine; I, isoleucine; S, serine; G, glycine; V, valine; A, alanine; L, leucine. Nucleotides: T, thymine; C, cytosine; G, guanine; A, adenine.