FIG. 2.

Decursin-induced apoptosis in B16F10 cells. (A) B16F10 cells were seeded into 60-mm plates and treated by decursin at various concentrations for 48 h and examined with a microscope. (B, C) One hundred micromolars of decursin was added for an indicated time and concentration. Apoptosis was detected using the annexin V assay for flow cytometry. Decursin caused an increase in transferase-mediated nick-end labeling (TUNEL) positive cell area with an increasing time of treatment. After 48 h of treatment, cells were stained using TUNEL or DAPI. Representative image of TUNEL and DAPI staining at 400×magnification from the phosphate-buffered saline (PBS)-treated and 10 mg/kg decursin-treated groups are shown in (D). (E) The graph showed the average number of fluorescence dots in images from each treatment group. Original microscope magnification=200×. *P < .05, **P < .01. Color images available online at www.liebertpub.com/jmf