FIG. 4.

Decursin inhibits tumor growth in a B16F10 tumor model. (A) C57BL/6 mice with B16F10 melanomas were injected with 10 mg/kg of decursin (n=6), or PBS (n=6) for 10 alternate days. The tumor size was measured every other day and the tumor volume was calculated. (A) Whole tumor tissues at 20 days of implantation. (B) This graph indicates the tumor weights after 10 mg/kg decursin treatment. (C) Tumors after 20 days of implantation were cut into sections and immunofluoresence staining was performed. Representative image of TUNEL and DAPI staining at 400×magnification from the PBS-treated and 10 mg/kg decursin-treated groups are shown in (C). (D) Decursin induced apoptosis and modulated the protein expression of procaspase-3 and beta-actin in B16F10 tumor tissues. Representative immunoblots for phosphorylated procaspase-3 and actin in tumor tissue isolated from PBS- and decursin-treated mice. *P < .05, **P < .01. Color images available online at www.liebertpub.com/jmf