Skip to main content
. 2015 Jun 16;21(10):995–1004. doi: 10.1089/ten.tec.2014.0699

FIG. 2.

FIG. 2.

Time course of changes in Eln endogenous fluorescence and second harmonic generation (SHG) of collagen in mouse EBs. (A) Z-projections of 16 z-stack multiphoton images showing endogenous fluorescence of Eln (top row: ex 780 nm/em 520 nm) and corresponding SHG of collagen (bottom row: ex 890 nm/em 445 nm) in EBs at different developmental stages. Arrows show the formation of fine Eln fibers in the day 12 EBs. Scale bar=100 μm. (B) Quantification of Eln endogenous fluorescence intensity during EB development. (C) Quantification of SHG intensity during EB development. Bars indicate p<0.05. AIU, arbitrary intensity units.