Figure 2. IL-1α is released after genotoxic stress in the absence of detectable processing or cell integrity breakdown.

Total cell lysates from human HT1080 fibrosarcoma exposed to (a) bleomicin or (b) H₂O₂ were assessed for processing of precIL-1α. For this total proteins were extracted from cell pellets at indicated time points and separated over 15% SDS PAGE. IL-1α or γH2AX western blots were preformed. Histones were used as loading control. (c) Detection of necrosis by release of lactate dehydrogenase activity. HT1080 fibrosarcoma were exposed to several genotoxic stresses as above (UV, H₂O₂ and bleomycin). Supernatants were collected and analyzed for release of lactate dehydrogenase. Data is expressed as mean ± SD of 3 independent experiments. (d) Determination of mono- and oligonucleosomes released by necrotic cell death in treated (as in Fig. 2c) HT1080 fibrosarcoma or HaCaT keratinocytes. Data is expressed as mean relative absorbance (A_405 nm−A_490 nm) ± SD of 3 independent experiments.