FIGURE 1.
Effects of gaseous 1-pentanol, 2-pentanol, and 3-pentanol on Arabidopsis systemic resistance. (A,B) Arabidopsis seedlings were planted in an I-plate, which contains two physically separated compartments that share the same headspace. Plants are exposed to volatile 3-pentanol and its isoforms (or sterile distilled water control), which evaporates from solutions in the other compartment. All plants were drop-inoculated with P. syringae pv. tomato DC3000 (Pto) 7 days after exposure to volatiles from 1 nM, 100 nM, 10 μM, and 1 mM 1-pentanol, 2-pentanol, and 3-pentanol. Disease severity (B) was measured 7 days after pathogen inoculation. The asterisk in (B) indicates significant differences (P = 0.05 by LSD) between treatment and control. Error bars represent means ± SEM; N = 12 plants per treatment.