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. 2015 Oct 6;5:14784. doi: 10.1038/srep14784

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(A) The effect of 20E treatment on the expression of HaMCO1 transcript. (B) qPCR analysis of ECR and USP dsRNAi efficiency on ECR and USP transcript levels, respectively. (C) The effect of ECR and USP knockdown on HaMCO1 transcript level. The 18S rRNA was used as the housekeeping gene for normalization in all qPCR analyses. The data represent the mean ± SD of three biological replicates. The significance of comparisons were determined by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001).