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. 2015 Oct 6;6:216. doi: 10.3389/fphar.2015.00216

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Copyright © 2015 Hotchkiss, Berrigan and Pelis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA expression (A) and cell surface protein expression (B) of Organic anion transporter 2 (OAT2)-tv1 (tv1) or OAT2-tv2 (tv2) in Chinese hamster ovary (CHO), human embryonic kidney (HEK), or Madin-Darby canine kidney (MDCK) cells stably transfected with the respective construct, or in parental cells (P). mRNA expression was determined by reverse transcription polymerase chain reaction (RT-PCR) using oligonucleotide primers against OAT2. Amplified products were separated on 1% agarose gels and stained with ethidium bromide. Sulfo-NHS-SS-biotin was used for cell surface biotinylation.