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. 2015 Oct 6;6:1100. doi: 10.3389/fmicb.2015.01100

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Copyright © 2015 Dong, Zou, Liu, Yang, Huang, Liu, He, Liu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PSR assay specificity. Specificity of the PSR method for detecting Pseudomonas aeruginosa by real-time turbidimeter (A) or HNB colorimetric assay (B). Amplification was performed at 65°C for 60 min. 1, P. aeruginosa ATCC 15442; 2, P. aeruginosa CMCC 10539; 3, P. fluorescens CGMCC 1.1802; 4, Burkholderia pseudomallei 029; 5, P. geniculate CGMCC 1.871; 6, P. mendocina CGMCC 1.593; 7, P. putida 2309; 8, Klebsiella pneumoniae ATCC 2146; 9, Streptococcus pneumoniae 112-07; 10, Mycobacterium tuberculosis 005; 11, Staphylococcus aureus 2740; 12, Acinetobacter baumannii 12101; 13, Escherichia coli 44825; 14, Shigella flexneri 4536; 15, Stenotrophomonas maltophilia K279a; 16, Legionella pneumophila 9135; 17, Haemophilus influenza ATCC 49247; 18, Salmonella typhi 9275; 19, Proteus vulgaris CMCC 49027; 20, negative control (double distilled water).