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PSR assay sensitivity. Comparative sensitivities of the PSR assay (A) and (B) and traditional PCR (C) for detection of the P. aeruginosa toxA gene. 1–7: 10-fold serial dilution of genomic DNA extracted from P. aeruginosa ATCC 15442 (23.0 ng/μl to 0.023 pg/μl); 8: negative control (double-distilled water). The expected PCR product size was 396 bp.
