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. 2015 Oct 6;6:1072. doi: 10.3389/fmicb.2015.01072

FIGURE 4.

FIGURE 4

The coupling of NH3 oxidation to NO2- reduction in Methylomicrobium album strain BG8 under hypoxia. Experiments were performed in a closed 10 mL micro-respiratory chamber outfitted with an O2 and N2O microsensor and logged with Sensor Trace Basic software. O2 (black diamonds), N2O (gray circles), NO2- (black dashed triangles). Cells of M. album strain BG8 were grown and harvested as described in the materials and methods and resuspended in nitrogen free mineral salts medium. Arrows mark the addition of NH4+ (100 μM) to the closed micro-respiratory chamber. Traces (O2 + N2O) are single representatives of reproducible results from cultures grown on different days. NO2- was measured using a colorimetric method as described in the Section “Materials and Methods” and data points represent the mean ± SD for three technical replicates.