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. 2015 Oct 6;5:14601. doi: 10.1038/srep14601

Figure 5. Colocalization analyses of aggregated polyhedrin with HSC/HSP70s, ubiquitinated proteins and mitochondria.

Figure 5

Infected cells expressing polyhedrin-mCherry and polyhedrin-eGFP were collected at 24 h p.i., treated with paraformaldehyde and Triton X-100, and then immunostained for HSC/HSP70s (A) and ubiquitin (B). The mitochondria (C) were stained with a MitoTracker® deep red probe (Molecular Probes, Life technologies, Eugene, OR, USA). Cell nuclei were stained with Hoechst 33342. Cells were imaged under a Leica TCS SP5 confocal laser-scanning microscope.