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. 2015 Sep 17;12(12):2131–2142. doi: 10.1016/j.celrep.2015.08.053

Figure 3.

Figure 3

The S. marcescens T6SS Does Not Require Cell-Cell Contact for Activation and Does Not Exhibit “Dueling” Behavior

(A and B) Analysis of S. marcescens Db10 expressing the TssB-mCh reporter fusion together with uniform cytoplasmic GFP. (A) Part of a representative field of view showing the red fluorescence channel (TssB-mCherry), green fluorescence channel (constitutive cytoplasmic GFP), merged red/green, and the automatically generated GFP mask. Manually identified TssB-mCh foci are highlighted with white arrows; scale bar, 1 μm. (B) Percentage of cells with 0, 1, or 2+ foci, either within the whole population (all cells) or within the subgroup of isolated cells with no touching neighbors (single cells).

(C) T6SS-mediated killing of wild-type (T6SS+) or ΔtssE (T6SS mutant) S. marcescens ATCC274 target cells by different attacker strains of S. marcescens Db10, as indicated. WT, wild-type; ΔtssH, T6SS inactive mutant; ΔT6SS, mutant lacking entire T6SS; none, media only; points show mean ± SEM (n = 4).