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. 2015 Sep 17;12(12):2131–2142. doi: 10.1016/j.celrep.2015.08.053

Figure 6.

Figure 6

Quantitative Co-localization Analysis Demonstrates Non-reciprocal Preferences for TssH Foci to Co-localize with TssB Foci and for TssB Foci to Co-localize with TssL Foci

(A) Fluorescence microscopy images (TssB-GFP, left; TssX-mCh, middle) and map of automatically detected foci (right) of a representative field of view for S. marcescens co-expressing TssH-mCh and TssB-GFP (upper) or TssL-mCh and TssB-GFP (lower). On the map, GFP foci are shown in green and mCherry in red over the top. A small region of each map is magnified in the inset. Scale bars, 2 μm.

(B) Frequency histograms of the distances between TssH-mCh or TssL-mCh foci and their nearest TssB-GFP foci (left), or between TssB-GFP foci and their nearest TssH-mCh or TssL-mCh (right); cumulative data from 11 (TssH) or 10 (TssL) fields of view; bars show mean ± SEM. A dashed line indicates the 0.272-μm colocalization threshold.

(C) Overall frequencies of co-localization (foci < 0.272 μm apart) of TssH and TssL foci with TssB foci, or vice versa.

(D) Venn diagram summarizing the observed co-localization between TssB and TssH (left), or between TssB and TssL (right); the total number of TssH and TssL foci relative to number of TssB foci is given as a ratio.