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. 2014 Jun 17;6(2):e29236. doi: 10.4161/isl.29236

Figure 2.

Figure 2.

PAX4 overexpression blocks glucagon expression. (A) 21 d differentiated hESC cultures uninfected or infected with either Ad eGFP or Ad PAX4 at an MOI of 6 or 60 were assessed for expression of a number of targets by RT-qPCR relative to adult human islets. * Indicates significant overexpression of human PAX4 compared with control virus (Ad eGFP) at the same dose (P < 0.05 by 1-way ANOVA with Bonferroni post-hoc test). (B) Twenty-four hour static media samples were taken between days 11 and 21 were assayed by radioimmunoassay for glucagon and C-peptide content. (C) Nineteen day differentiated hESCs were tested for glucose regulated glucagon and C-peptide release in response to 1 h incubations in low glucose (2 mM), high glucose (25 mM), and potassium chloride (30 mM).