Table 1. RT-qPCR primers. Primers, product sizes and applicable references for transcripts tested in this study.
Gene Name | Gene Accession | Product Size (bp) |
Primer Sequence Forward / Reverse 5′→3′ |
Reference |
Insulin | NM_000207.2 | 245 | AGCCTTTGTGAACCAACACC GCTGGTAGAGGGAGCAGATG |
36 |
Glucagon | NM_002054.4 | 275 | CATTCACAGGGCACATTCAC CGGCCAAGTTCTTCAACAAT |
36 |
Somatostatin | NM_001048.3 | 126 | AGCTGCTGTCTGAACCCAAC CCATAGCCGGGTTTGAGTTA |
36 |
PAX4 | NM_006193 | 169 | AGCAGAGGCACTGGAGAAAGAGTT CAGCTGCATTTCCCACTTGAGCTT |
36 |
ARX | NM_139058.2 | 141 | CTGCTGAAACGCAAACAGAGGC CTCGGTCAAGTCCAGCCTCATG |
4 |
MAFA | NM_201589 | 195 | CTTCAGCAAGGAGGAGGTCA TTGTACAGGTCCCGCTCTTT |
4 |
HPRT | NM_000194.2 | 148 | TGTTGTAGGATATGCCCTTGACTAT GCGATGTCAATAGGACTCCAGA |
4 |