Table 2. Antibody Sources and Conditions for Immunocytochemistry Antibody concentrations, sources, and relevant staining condition as applicable for this study.
| Gene Name | Host Species |
Supplier / Catalogue number |
Staining Method | Dilution | Antigen Retrieval |
| PAX4 | Goat | R&D Systems AF2614 |
PFA fixed, paraffin section of agarose embedded cell pellet | 1:250 | HIER |
| eGFP | Mouse | Clontech 632375 |
PFA fixed, paraffin section of agarose embedded cell pellet | 1:500 | HIER |
| Synaptophysin | Rabbit | Novus NB120 - 16659 |
PFA fixed, paraffin section of agarose embedded cell pellet | 1:50 | HIER |
| Insulin | Guinea Pig | Sigma I8510 |
PFA fixed, paraffin section of agarose embedded cell pellet | 1:1000 | HIER |
| Glucagon | Rabbit | Cell Signaling 8233P |
PFA fixed, paraffin section of agarose embedded cell pellet | 1:500 | HIER |
| Somatostatin | Mouse | BCBC AB1985 |
PFA fixed, paraffin section of agarose embedded cell pellet | 1:1000 | HIER |
HIER (heat induced epitope retrieval): 15 min at 95 °C in 10mM Citrate buffer with 0.05% Tween-20 pH 6.0. BCBC (Beta Cell Biology Consortium)