Table 1.
Histochemical markers used for detection of extracellular matrix components, channel proteins, receptor proteins and transmitters
| Detected components | Antibodies/binding proteins | Dilution | Source | References | |
|---|---|---|---|---|---|
| Matrix constituents | IHC | WB | |||
| Aggrecan, core protein (aa 1177–1326; AB1031) | Rabbit anti‐aggrecan | 1:1000 | Millipore | Brückner et al. 2006 | |
| Aggrecan, core protein (HAG7D4) | Mouse anti‐human aggrecan | 1:10 | Serotec | (Brückner et al. 2008) | |
| Brevican (50 kDa cleavage fragment) | Rabbit anti‐human brevican | 1:2000 | R.T. Matthews | (Matthews et al. 2000) | |
| Neurocan, N‐terminal part (MAB 5212) | Sheep anti‐neurocan | 1:500 | 1:2500 | R&D Systems | (Milev et al. 1998) |
| Brevican (aa 232–394) | Mouse anti‐brevican | 1:1000 | BD Bioscience | (Vitellaro‐Zuccarello et al. 2007) | |
| Channel proteins | |||||
| Voltage‐gated calcium channel Cav1.2 | Rabbit anti‐Cav1.2 | 1:200 | Alomone Labs | (Lv et al. 2014) | |
| Voltage‐gated calcium channel Cav1.3 | Rabbit anti‐Cav1.3 | 1:200 | Alomone Labs | (Pirone et al. 2014) | |
| Voltage‐gated calcium channel Cav2.1 | Rabbit anti‐Cav2.1 | 1:200 | Millipore | (Mallmann et al. 2013) | |
| Voltage‐gated potassium channel Kv1.3 | Rabbit anti‐Kv1.3 | 1:300 | Alomone Labs | (Gazula et al. 2010) | |
| Voltage‐gated potassium channel Kv1.6 | Rabbit anti‐Kv1.6 | 1:400 | Alomone Labs | (Gazula et al. 2010) | |
| Voltage‐gated potassium channel Kv2.2 | Rabbit anti‐Kv2.2 | 1:500 | Alomone Labs | (Tong et al. 2013) | |
| Voltage‐gated potassium channel Kv3.1β | Rabbit anti‐Kv3.1ß | 1:800 | Alomone Labs | (Härtig et al. 1999) | |
| Voltage‐gated potassium channel Kv3.1β | Mouse anti‐Kv3.1ß | 1:300 | NeuroMab | (Tong et al. 2013) | |
| Receptor proteins | |||||
| C‐terminal part of GluR2 and GluR3 | Rabbit anti‐GluR 2/3 | 1:100 | Chemicon | (Thiers et al. 2008) | |
| AMPA receptor (GluR4) protein | Rabbit anti‐GluR4 | 1:500 | Cell Signaling | (Caicedo and Eybalin, 1999) | |
| NMDA receptor subunit NMDAR2A | Rabbit anti‐NMDAR2A | 1:1000 | Millipore | (Kim et al. 2008) | |
| Transmitters | |||||
| Glutamate transporter 1 | Guinea pig anti‐vGlut1 | 1:500 | Synaptic Systems | (Takamori et al. 2000) | |
| Glutamate transporter 2 | Guinea pig anti‐vGlut2 | 1:250 | Synaptic Systems | (Takamori et al. 2000) | |
| Glial Glutamate transporter 1, C‐terminal part (AB1783) | Guinea pig anti‐Glt‐1 | 1:1000 | Millipore | (Pines et al. 1992) | |
| Reference protein | |||||
| Heat shock protein 90 kDa | Rabbit anti‐HSP90 | 1:1000 | 1:1000 | Cell Signaling | (Suttkus et al. 2014) |