Figure 1.

qRT-PCR for the main enzymes and receptors of ECS in LLC-PK1 cells. (A) Detection of mRNA for the enzymes responsible for AEA (NAPE-PLD) and 2A-G (DGL-α and β) synthesis, respectively. The main enzymes for the degradation of AEA and 2-AG, FAAH and MGL, respectively, were also expressed. (B) LLC-PK1 cells also possess the mRNA for CB₁, CB₂ and TRPV1 receptors. Data are means ± SEM of n = 3; *Indicates different from control (P < 0.05). We also calculated the Power Test (Error Type II β), which was 99.9%.