Figure 2.

Immunofluorescence of CB₁ and CB₂ receptors, and TRPV1 channels. In all the panels, cell nuclei are marked in blue (DAPI), cannabinoid receptors are in green (Alexa 488) and Na⁺/K⁺-ATPase in red (Alexa 594). Rows are as follows (A) CB₁, (B) CB₂ and (C) TRPV1. Columns are as follows: first column is the negative control (no primary antibody incubation); the second shows the expression of each studied receptor; the third presents the Na⁺/K⁺-ATPase distribution; and the fourth is the merged image of each receptor (green) and Na⁺/K⁺-ATPase (red). Representative images from three different immunofluorescence assays for each receptor. Magnification 40×.