Figure 9.

Involvement of PKA and PKC in the modulation of Na⁺/K⁺ -ATPase activity induced byHP and WIN. (A) When indicated, cells were pretreated for 15 min with H89 (10⁻⁵ M), a PKA cell-permeable inhibitor, before the incubation with WIN (10⁻⁷ M) or HP (10⁻⁶ M). Na⁺/K⁺-ATPase activity was determined after 30 min incubation. Data are means ± SEM from different experiments (n = 6); *Indicates different from control (P < 0.05). (B) When indicated, cells were pretreated for 15 min with, calphostin C (10⁻⁸ M), a PKC inhibitor, before the incubation with WIN (10⁻⁷ M) or HP (10⁻⁶ M). Na⁺/K⁺-ATPase activity was determined after 30 min incubation. Data are means ± SEM from different experiments (n = 6); *Indicates different from control (P < 0.05).