Figure 10.

HP decreases ERK1/2 phosphorylation in LLC-PK1 cells. Whole cell homogenates were obtained from cultures treated with WIN (10⁻⁷ M), HP (10⁻⁶ M), in the absence or presence of the CB₁ antagonist AM251 (10⁻⁷ M) for 30 min, as depicted. SDS-PAGE and immunoblotting were performed according to Methods. (A) Representative image of pERK1/2 and ERK 1/2 immunodetection. (B) Graph shows the ratio between pERK/ERK after densitometric analysis of the chosen bands in the different experimental situations. Data are means ± SEM from different experiments (n = 6); *Indicates different from control (P < 0.05).