Table 2.
WTC-01 induced early apoptosis in two human NPC cell lines
| NPC-TW01 | HONE-1 | |||||
|---|---|---|---|---|---|---|
| Hours | E | L | E + L | E | L | E + L |
| 0 | 0.55 | 0.06 | 0.51 | 0.21 | 0.04 | 0.25 |
| 6 | 0.91 | 0.03 | 0.94 | 0.33 | 0.01 | 0.34 |
| 12 | 12.52 | 5.78 | 18.30 | 4.72 | 1.03 | 5.75 |
| 18 | 14.37 | 11.99 | 26.36 | 8.04 | 1.23 | 9.27 |
NPC cells were treated with 0.45 μM of WTC-01 for the period indicated then WTC-01-treated cells were harvested, incubated with Annexin V and PI and subjected to flow cytometric analysis. Data shown are from a single experiment and indicate the population percentage of each stage. E, early phase apoptosis; L, late phase apoptosis.