Figure 2.

The induction of haemoglobin expression in primary astrocytes and neuron-like PC12 cells by EH-201 is mediated by EPO. (A, D) rhEPO treatment for 24 h increased haemoglobin-β mRNA expression in astrocytes and PC12 cells. (B) EH-201-treated primary astrocytes with or without the neutralizing EPO antibody (nEPO-ab, 1 μg·mL⁻¹) were analysed for haemoglobin-β expression. (E) EH-201 treatment for 24 h increased haemoglobin-β mRNA expression in PC12 cells. The expression of GAPDH was used as an internal control. The results are expressed as the relative index of untreated controls means ± SD from at least three independent measurements. *P < 0.05, **P < 0.01; significantly different from control; one-way anova followed by Tukey's multiple comparison test. (C, F) Different forms of haemoglobin (monomer: 16 kD, dimer: 32 kD, tetramer: 64 kD) expression identified by an haemoglobin-β Ab in primary astrocytes and PC12 cells treated with EH-201. The results are expressed as the relative expression of untreated controls ± SD from at least three independent measurements. *P < 0.05, **P < 0.01, significantly different from control; Student's t-test.