Table 10.
Steady-state slow inactivation | |||||
---|---|---|---|---|---|
Temperature | SSSI-V½ (mV) | SSSI-z | SSSI-y0 | n | |
Wild type | Q10 = 0.92 | Q10 = 0.88 | Q10 = 0.88 | ||
10°C | −103.81 ± 3.13 | −2.69 ± 0.07 | 0.28 ± 0.03 | 6 | |
22°C | −95.27 ± 4.04 | −2.27 ± 0.57 | 0.26 ± 0.04 | 4 | |
34°C | −85.76 ± 5.90 | −1.97 ± 0.58 | 0.40 ± 0.06 | 4 | |
37°C | −90.41 | −2.12 | 0.37 | — | |
41°C | −89.00 | −2.07 | 0.38 | — | |
R1193Q | Q10 = 0.95 | Q10 = 0.79 | Q10 = 1.03 | ||
10°C | −101.66 ± 3.20 | −3.13 ± 0.90 | 0.34 ± 0.03 | 5 | |
22°C | −94.76 ± 6.57 | −1.85 ± 0.24 | 0.30 ± 0.02 | 5 | |
34°C | −89.03 ± 7.45 | −1.96 ± 0.28 | 0.32 ± 0.06 | 4 | |
37°C | −92.03 | −1.96 | 0.31 | — | |
41°C | −91.02 | −1.87 | 0.30 | — | |
E1784K | Q10 n/a | Q10 n/a | Q10 n/a | ||
10°C | −117.02 ± 1.90 | −2.27 ± 0.21 | 0.26 ± 0.04 | 6 | |
22°C | −114.15 ± 7.09 | −2.31 ± 0.43 | 0.32 ± 0.06 | 5 | |
34°C | n/a | n/a | n/a | — | |
37°C | n/a | n/a | n/a | — | |
41°C | n/a | n/a | n/a | — |
Values in bold were determined by extrapolation of Q10 fits to measured values.