Table 5.
Fast inactivation time constants (ms) | |||||||||
---|---|---|---|---|---|---|---|---|---|
Temperature | −50 mV | n | −30 mV | n | −10 mV | n | +10 mV | n | |
Wild type | Q10 = 5.80 | Q10 = 2.13 | Q10 = 1.80 | Q10 = 1.79 | |||||
10°C | 24.40 ± 4.86 | 4 | 7.39 ± 0.48 | 9 | 4.96 ± 0.29 | 9 | 4.34 ± 0.29 | 9 | |
22°C | 20.10 ± 5.11 | 5 | 1.98 ± 0.29 | 8 | 0.96 ± 0.10 | 8 | 0.67 ± 0.09 | 8 | |
34°C | 2.09 ± 0.59 | 5 | 0.88 ± 0.16 | 8 | 0.61 ± 0.13 | 8 | 0.45 ± 0.09 | 8 | |
37°C | 1.19 | — | 0.69 | — | 0.49 | — | 0.36 | — | |
41°C | 0.59 | — | 0.51 | — | 0.39 | — | 0.29 | — | |
R1193Q | Q10 = 2.02 | Q10 = 1.89 | Q10 = 1.90 | Q10 = 1.82 | |||||
10°C | 23.60 ± 3.69 | 4 | 7.08 ± 0.42 | 10 | 4.49 ± 0.35 | 10 | 3.76 ± 0.24 | 10 | |
22°C | 3.34 ± 0.54 | 6 | 1.29 ± 0.10 | 6 | 0.73 ± 0.03 | 6 | 0.53 ± 0.04 | 6 | |
34°C | 1.80 ± 0.27 | 11 | 0.75 ± 0.12 | 11 | 0.43 ± 0.07 | 11 | 0.35 ± 0.04 | 11 | |
37°C | 1.41 | — | 0.60 | — | 0.34 | — | 0.28 | — | |
41°C | 1.06 | — | 0.46 | — | 0.27 | — | 0.22 | — | |
E1784K | Q10 = 1.99 | Q10 = 2.03 | Q10 = 1.84 | Q10 = .84 | |||||
10°C | 8.59 ± 0.71 | 6 | 4.42 ± 0.36 | 8 | 3.47 ± 0.26 | 8 | 3.20 ± 0.21 | 8 | |
22°C | 1.73 ± 0.23 | 10 | 0.90 ± 0.12 | 10 | 0.58 ± 0.06 | 10 | 0.49 ± 0.05 | 10 | |
34°C | 0.91 ± 0.18 | 10 | 0.45 ± 0.07 | 10 | 0.36 ± 0.05 | 10 | 0.31 ± 0.05 | 10 | |
37°C | 0.72 | — | 0.36 | — | 0.29 | — | 0.25 | — | |
41°C | 0.54 | — | 0.27 | — | 0.23 | — | 0.19 | — |
Values in bold were determined by extrapolation of Q10 fits to measured values.