Table 9.
Slow inactivation onset | |||||
---|---|---|---|---|---|
Temperature | SIonset – y0 | SIonset – τ1 (s) | SIonset – τ2 (s) | n | |
Wild type | Q10 = 0.95 | Q10 = 0.05 | Q10 = 0.99 | ||
10°C | 0.28 ± 0.03 | 0.15 ± 0.04 | 18.71 ± 4.51 | 6 | |
22°C | 0.26 ± 0.04 | 5.62 ± 1.99 | 22.25 ± 6.82 | 5 | |
34°C | 0.32 ± 0.05 | 2.49 ± 0.84 | 19.31 ± 6.30 | 5 | |
37°C | 0.30 | n/a | 20.40 | — | |
41°C | 0.31 | n/a | 20.51 | — | |
R1193Q | Q10 = 1.13 | Q10 = 0.10 | Q10 = 0.97 | ||
10°C | 0.28 ± 0.05 | 0.12 ± 0.06 | 16.35 ± 5.15 | 5 | |
22°C | 0.29 ± 0.05 | 2.14 ± 1.00 | 14.97 ± 3.96 | 6 | |
34°C | 0.21 ± 0.03 | 1.68 ± 0.35 | 17.61 ± 2.33 | 5 | |
37°C | 0.21 | n/a | 17.00 | — | |
41°C | 0.20 | n/a | 17.22 | — | |
E1784K | Q10 = 1.07 | Q10 = 0.12 | Q10 = 1.40 | ||
10°C | 0.26 ± 0.07 | 0.26 ± 0.05 | 18.48 ± 1.80 | 4 | |
22°C | 0.24 ± 0.05 | 4.02 ± 1.73 | 13.71 ± 2.38 | 5 | |
34°C | 0.22 ± 0.05 | 2.89 ± 1.02 | 8.61 ± 4.13 | 5 | |
37°C | 0.22 | n/a | 7.82 | — | |
41°C | 0.21 | n/a | 6.83 | — |
Values in bold were determined by extrapolation of Q10 fits to measured values.