B. burgdorferi levels resurge in tissues at 12 months after antibiotic treatment. Copy numbers of B. burgdorferi flab DNA, determined by quantitative PCR, in ear, inoculation site (Inoc), heart base (HB), ventricular muscle (VM), quadriceps muscle (QM), and tibiotarsus (Tt) tissues of saline- and antibiotic-treated mice at 12 months after treatment [149].