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. 2015 Apr 29;8(4):200–205. doi: 10.1080/19336934.2015.1045171

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© 2014 Taylor & Francis Group, LLC

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Figure 2. — Characterization of the aggression-modulating serotonergic PLP neurons. (A) 5HT-PLP neurons do not co-express Fru^M. Anti-Fru^M staining is shown in magenta, serotonergic neurons are visualized by TRH-Gal4/UAS-CD8:GFP (green). The full z stack frontal projection is shown. A' shows the optical section through the 5HT-PLP cell bodies (white arrows). (B) 5HT-PLP neurons do not co-express Dsx. Anti-5HT staining is shown in magenta, Doublesex-positive neurons are visualized by Dsx-Gal4/UAS-CD8:GFP. The full z stack frontal projection is shown. B' shows the optical section through the 5HT-PLP cell bodies (white arrows). (C) 5HT-PLP neurons have similar morphology in males and females. Individual 5HT-PLP neurons are visualized by TRH-Gal4/UAS>stop>CD8:GFP in combination with 417-FLP. (D) 5HT-PLP neurons do not co-express NPF. Anti-5HT staining is shown in magenta, Neuropeptide F-positive neurons are visualized by NPF-Gal4/UAS-CD8:GFP. The anterior and posterior z stack frontal projections are shown separately. White arrows point to the 5HT-PLP cell bodies (posterior view).