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. 2014 Oct 31;8(5):402–413. doi: 10.4161/19336950.2014.950537

Table 1.

Effect of MLCK inhibition on Ca2+ signaling in cultured or freshly isolated cells

Cell type MLCK Inhibition Stimulating agent Effect on [Ca2+]cyt References
Freshly isolated rabbit portal vein  myocytes ML-9 (5 μM), ML-7 (5 μM), MLCK(11-19)amide (5 μM) NA, GTPγS, OAG ↘Icat 43
  AV25 (10 μM)   No effect on Icat(ATP)  
Freshly isolated rabbit mesenteric  artery myocytes WT (20 μM) AngII ↘Icat 122
    OAG No effect  
Freshly isolated guinea-pig gastric  myocytes ML-7 (3 μM) CCh ↘ICCh 50
    Voltage ↘IBa (L-type VOC)  
Freshly isolated human  monocytes/ macrophages ML-9 (10-100 μM), WT (1-100 μM), MLCK antisense TG, CPA ↘CCE 44
Freshly isolated murine ileal  myocytes ML-7 (5 μM), ML-9 (10 μM) CCh ↘ICCh 42
Cultured bovine adrenal  glomerulosa cells WT (10 μM), ML9 (100 μM), KT5926 (100 μM), MS-347a (30 μM) AngII ↘Ca2+ entry 123
    Iono, TG, KCl No effect  
Cultured porcine aortic  endothelial cells ML-9 (1-100 μM) BK, TG ↘CCE 124,125
  ML-7 (0.1-30 μM)     126
  MLCK antisense     41
Cultured rat pulmonary artery  endothelial cells ML-9 (100 μM) TG ↘CCE 127
Cultured rat pulmonary artery  smooth muscle cells ML-9 (10-100 μM), ML-7 (1-100 μM) Hypoxia ↘CCE 128
Cultured human HEK293 cells ML-9 (100 μM) TG ↘CCE;
↘ICRAC 		49
  MLCK siRNA, WT (20 μM)   No effect  
Cultured human HEK293 cells  overexpressing TRPC5 ML-7 (3 μM), MLCK-siRNA CCh ↘ICCh 46
Cultured human HEK293 cells  overexpressing TRPC5 ML-9 (3-50 μM), WT (1-10 μM), CCh, ATP ↘Ca2+ entry (no effect on Ca2+ release) 45
  MLCK dominant negative   ↘ ICRAC  
Cultured human HEK293 cells  overexpressing TRPC6 ML-9 (10 μM), ML-7 (10 μM) CCh ↘ICCh 48
  WT (3 μM), MLCK dominant negative inhibitory peptide   No effect  
Cultured rat aortic SMC ML-7 (10 μM) VP No effect 47
Cultured rat aortic cell line A7r5 MLCK-siRNA VP ↘Ca2+ entry 410
    Voltage ↘IBa (L- and T-type VOC)  

Abbreviations: NA: noradrenaline; VP: vasopressin; KCl: physiological solution enriched in K+; TG: thapsigargin; Iono: ionomycin; AngII: angiotensinII; BK: bradykinin; CCh: carbachol; CPA: cyclopiazonic acid; WT: wortmannin; OAG: 1-oleoyl-2-acetyl-sn-glycerol ; ↘ decrease; VOC: voltage-dependent Ca2+ channels; IBa: barium current; ICRAC: calcium-release activated calcium current; Icat: cationic current; CCE: capacitative Ca2+ entry.