Table 3.
Effect of MLCK inhibition on Ca2+ signaling in smooth muscle tissues
| Tissue type | Inhibitor | Stimulating agent | Effect on [Ca2+]cyt | References |
|---|---|---|---|---|
| Rat aorta | WT (1 μM) | KCl, Phe | No effect | 52 |
| Rabbit aorta | WT (1 μM) | KCl, DPB | No effect | 53 |
| Rat aorta | ML-7 (3 μM) | NA | ↘Ca2+ influx | 47 |
| Rat resistance mesenteric artery | ML-7 (3 μM), MLCK-siRNA | NA, KCl | ↘Ca2+ influx; ↘IBa (L-type VOC) |
10 |
| Porcine carotid artery | ML-9 (10-100 μM), WT (10 μM) | KCl | ↘Ca2+ influx | 56 |
| Porcine aortic endothelial cells (in valvular strips) | ML-9 (30-100 μM) | TG | ↘CCE | 131 |
| WT (10 μM) | No effect | |||
| Guinea-pig tracheal smooth muscle | ML-9 (10-100μM) | KCl, MC, TG | ↘Ca2+ influx | 57 |
| WT (3 μM) | No effect | |||
| Guinea-pig ureter | WT (4 μM) | KCl | No effect | 54 |
| Human myometrium | WT (4 μM) | Spontaneous contraction, oxytocin | No effect | 55 |
Abbreviations: NA: noradrenaline; VP: vasopressin; KCl: physiological solution enriched in K+; DPB: 12-deoxyphorbol 13-isobutyrate; MC: methacholine; TG: thapsigargin; Phe: phenylephrine; WT: wortmannin; ↘ decrease; IBa: barium current; CCE: capacitative Ca2+ entry.