Figure 1.

Tamoxifen-induced activation of Cre recombinase in CGN. (A–C) X-Gal (blue) and nuclear fast red (red) stained cerebelli of a R26R control and 2 Math1-Cre/R26R mice at P12 after 3 tamoxifen injections during early postnatal development. As indicated by the red boxes in A, high magnification (right micrograph) shows a section of folium 4 that was used for further analyses throughout this study. Active Cre, as indicated by blue X-Gal staining, is evident in all 3 layers of the cerebellar cortex, namely the external granule cell layer (EGL), the molecular layer (ML) and the internal granule cell layer (IGL). Scale bars in C correspond to 250 μm (left), 100 μm (middle) and 100 μm (right).