Figure 3.

Impaired CGN radial migration in Pfn1^{flx/flx,Math1-Cre} mice. (A and B) BrdU-positive cells (green) in folia 4 of a Pfn1^flx/flx control and a Pfn1^{flx/flx, Math1-Cre} mouse 96 h after a BrdU injection at P8. Sections were counterstained with propidium iodide (PtdIns, red). Scale bar in A corresponds to 50 μm. (C) Compared to Pfn1^flx/flx controls, the migration distance of BrdU-positive cells is decreased in Pfn1^{flx/flx,Math1-Cre} mice, as it is obvious from the right shift of the cumulative curve and the reduction in the mean migration distance (inset). (D) Distribution of BrdU-positive cells within the cerebellar cortex. Equal numbers were found in the EGL, whereas the relative number of BrdU-positive cells was increased in the ML and decreased in the IGL of Pfn1^{flx/flx,Math1-Cre} mice. ns: not significant, **: P < 0.01, ***: P < 0.001.