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. 2015 Oct 6;9:388. doi: 10.3389/fncel.2015.00388

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Copyright © 2015 Zhang, Song, Gu, Ren, Verkhratsky and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of fluoxetine on gene expression of 5-HT_2B receptor and behavior changes of anhedonia mice. After either 7 days or 14 days (7 days after the end of MPTP treatment) of MPTP treatment, mice with anhedonia were daily injected intraperitoneally with fluoxetine (10 mg/kg/d dissolved in 0.9% NaCl) or saline for 14 days. (A) mRNA expression of 5-HT_2B receptor was measured by RT-PCR. A representative experiment showing mRNA for 5-HT_2B receptor and for TBP, as a housekeeping gene. The size of PCR product of 5-HT_2B receptor is 370 bp, and that of TBP 236 bp. Similar results were obtained in three independent experiments. Average mRNA expression was quantified as ratios between 5-HT_2B receptor and the housekeeping TBP gene. Ratios between 5-HT_2B receptor and TBP in control group were designated a value of one. SEM values are indicated by vertical bars. *Indicates statistically significant (p < 0.05) difference from other groups. (B) Protein level measured by immunoblotting of 5-HT_2B receptor (55 kDa). Immunoblots from representative experiments. Bands of 55 and 42 kDa represent 5-HT_2B receptor and β-actin, respectively. Similar results were obtained in three independent experiments. Average protein level was quantified as ratios between 5-HT_2BR and β-actin. Ratios between 5-HT_2B receptor and β-actin in control group were designated a value of one. SEM values are indicated by vertical bars. *Indicates statistically significant (p < 0.05) difference from other groups. (C) Percentage of sucrose preference. Values were expressed as the mean ± SEM (7 days, n = 5; 14 days, saline: n = 6, MPTP: n = 6, flu: n = 5, MPTP plus flu: n = 6). *Indicates statistically significant (p < 0.05) difference from other groups at the same treatment period. (D) Forced-swimming test. The values of immobility time are expressed as the mean ± SEM (7 days, n = 5; 14 days, saline: n = 6, MPTP: n = 6, flu: n = 5, MPTP plus flu: n = 6). *Indicates statistically significant (p < 0.05) difference from other groups at the same treatment period.