Figure 2.

Rasd2 and Rit1 activates TRPC4 channel. (A) All panels indicate I-V relationship of currents measured from HEK293 cells expressing mouse TRPC4β-ECFP channel and constitutively active Rasd2^S40V or Rit1^Q79L proteins. We studied TRPC4 channel activity with Cs⁺-rich extracellular solution (Cs) since TRPC4 has greater permeability to Cs⁺ than Na⁺. Rasd2^S40V and Rit1^Q79L proteins activated TRPC4 channel without GTPγS. (B) Summarized current density measured above. Rasd2^S40V or Rit1^Q79L proteins induced TRPC4 current increase. The comparison between pECFP and Rasd2^S40V or Rit1^Q79L was carried out with Student's t-test. Statistical significance was denoted by an asterisk (*) at P< 0.05.