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. 2014 Oct 31;8(5):433–443. doi: 10.4161/19336950.2014.949188

Figure 1.

Figure 1.

Bright-field (A) and epifluorescent (B) illumination of a heterotypic Cx40/Cx43 N2a cell pair after membrane labeling with and 24 hours in culture. (C) Western blots for Cx40, Cx43, and Cx45 protein expression in parental N2a (left lane), stable rat Cx40-N2a clone (left center lane), stable rat Cx43-N2a clone (right center lane), or transiently expressed murine Cx45 in parental N2a cells (right lane) relative to GAPDH. No endogenous Cx40, Cx43, or Cx45 expression was detected with picomole sensitive detection kit. (D) Endogenous Cx40, Cx43, and Cx45 was detectable with femtomolar detection. E, Real-time PCR results for endogenous murine Cx45 or exogenous rat Cx40 or Cx43 mRNA expression in parental or stable Cx N2a cell clones relative to GAPDH. Exogenously expressed Cx43 or Cx40 mRNA levels were 0.6–1.0 times the GAPDH level in their respective stable cell clones whereas endogenous Cx mRNA levels were <0.001 in all parental and stable N2a cell clones.