Figure 4.

(A) Single gap junction channel currents (i_j) recorded from one heterotypic Cx43/Cx40 cell pair depicting a 120 pS channel at V_j = +50 mV and 95 pS channel at −50 mV relative to the Cx40 cell. (B) A different Cx43/Cx40 heterotypic cell pair exhibited mostly 50 or 70 pS channel conductance (γ_j) states at ±40 mV relative to the Cx40-expressing cell. (C) Summarized γ_j data from 8 cell pairs in a frequency histogram and multi-gaussian curve fits suggested 2 peaks at approximately 51 pS and 90 pS (N = 237 events). (D) The average i_j – V_j relationship for 4 Cx43/Cx40 cell pairs and linear curve fits for all channel events above or below 65 pS in amplitude. These pooled channel events averaged 52 or 94 pS, indicative of Cx43 γ_j values.