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. 2015 Oct 2;56(11):6196–6207. doi: 10.1167/iovs.15-16795

Figure 3.

Figure 3

Overexpression of ERp29 protects HRPE cells from CSE-induced apoptosis and cell death. ARPE-19 cells were treated with Ad-ERp29 (20 MOI) to induce ERp29 overexpression or Ad-GFP as control, and then exposed to 320 μg/mL CSE for 24 hours. (A) Protein level of cleaved caspase-3 was determined by Western blot analysis. Left: Western blot images, right: densitometry results normalized with β-actin. (B) Apoptosis was examined by the TUNEL assay. Left: TUNEL assay images. Scale bars: 100 μm. Right: Quantification of TUNEL-positive cells. (C) Dead cells were detected using in situ trypan blue staining. Left: Images of trypan blue staining. Scale bars: 100 μm. Right: Quantification of trypan blue staining. All data are expressed as the mean ± SD from three independent experiments. **P < 0.01 versus Ad-GFP; ##P < 0.01 versus Ad-GFP + CSE.