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. 2015 Oct 2;56(11):6196–6207. doi: 10.1167/iovs.15-16795

Figure 5.

Figure 5

Knockdown of ERp29 exacerbates CSE-induced apoptosis and cell death in human RPE cells. ARPE-19 cells were transfected with siRNA targeting ERp29 (Si-ERp29) or Ctrl siRNA (Si-Ctrl), then treated with 320 μg/mL CSE for 24 hours. (A) Protein levels of cleaved caspase-3 and cleaved PARP were determined by Western blot analysis. Right: Western blot images. Left: Densitometry results normalized with β-actin. (B) Apoptosis of ARPE-19 cells was examined by the TUNEL assay. Left: TUNEL assay images. Scale bars: 100 μm. Right: Quantification of TUNEL-positive cells. (C) Dead cells were detected using trypan blue staining. Left: Images of trypan blue staining. Scale bars: 100 μm. Right: Quantification of trypan blue staining. All data are expressed as the mean ± SD from three independent experiments. **P < 0.01 versus Si-Ctrl; ##P < 0.01 versus Si-Ctrl + CSE.