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. 2015 Aug 31;8(4):e1056950. doi: 10.1080/19420889.2015.1056950

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Published with license by Taylor & Francis Group, LLC

© Masaaki Iwamoto, Yasushi Hiraoka, and Tokuko Haraguchi

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — De novo assembly of the macronuclear NPC occurs only in presumptive new macronuclei. (A) Schematic representation of nuclear differentiation of Tetrahymena. NPC type switching from MIC-type (represented by blue) to MAC-type (represented by red) occurs only in the two anterior-located daughter nuclei of the second postzygotic division (PZD) of the MIC-like zygotic nucleus in each mating partner. The two nuclei that acquire MAC-type NPCs differentiate to new macronuclei. (B) Localization of fluorescent protein-tagged nucleoporins early in nuclear differentiation. Methanol-fixed mating pairs were observed. MacNup98B (representing the MAC-type NPC) appeared only in the anterior daughter nuclei of the second PZD (left panel). The pre-existing MicNup98A (representing the MIC-type NPC) is evenly segregated to both daughter nuclei (center panel). Nup93 that commonly exists in all NPCs (representing both types of NPC) exhibits biased distribution to the anterior nuclei (right panel). Left and center panels show the same mating partner of the pair expressing GFP-MacNup98B and mCherry-MicNup98A. mCherry fluorescence in the center panel is shown in green. In the right panel, the single partner of the GFP-Nup93-expressing pair is different from that shown in the other panels. In all panels, the upper side is the anterior of the cell and magenta represents DAPI staining. Bar is 10 mm.