Figure 3.
Rac1 inhibitor antagonizes Snail-mediated cell migration. 22Rv1 Snail-overexpressing cells were treated with NSC23766, Rac1 inhibitor, at 50 µM, 100 µM and 200 µM for 24 hrs. (A) Rac1 activity was assayed using G-LISA assay and (B) western blot analysis was performed to show the protein levels of Rac1, Snail, maspin, total and phospho-AKT and ERK and actin as a loading control. (C) Cell migration through collagen was performed using the boyden chamber for 24 hrs and represented as images taken by light microscopy as well as graphing of cell numbers following counting. Results are reported as mean ± SD from 2 independent experiments done in triplicates and protein gel blots done as 3 independent experiments. Statistical significance was assessed using GraphPad Prism software by paired Student's t-test compared to 22Rv1 Snail control cells treated with ddH2O (#,*P < 0.05, **P < 0.01, ***P < 0.001).