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. 2014 Dec 23;7(6):e994969. doi: 10.4161/19420889.2014.994969

Figure 2.

Figure 2.

MICALL1 is required for Tfn-positive endosomal tubules formation in human dendritic cells. (A) MICAL-L1 RNA expression is efficiently silenced after 36 hrs by 3 unique 27 mer siRNA targeting MICAL-L1 (gray bars) and not by scrambled control siRNA (black bars). (B) Confocal image of stimulated moDCs (200 ng/ml LPS, 5 ug/ml polyI:C, and 3 ug/ml pp65 antigen) 36 hours after 10 nM siRNA treatment (left, control siRNA; right, MICALL1 siRNA). Prior to (upper part) and 60-80 minutes post (lower part) addition of antigen-specific CD8+ T cells. Red depicts LDL, Green Transferrin, and yellow co-localization of LDL and Tfn. (C) The percentage of stimulated moDCs (200 ng/ml LPS, 5 ug/ml polyI:C, and 3 ug/ml pp65 antigen) with LDL+ endosomal tubules expressing tubular Tfn+ endosomes upon antigen-specific CD8+ T cells, 36 hrs after indicated siRNA treatment (10 nM scrambled or MICALL1-targeting siRNA). Data represents mean ± SE of three independent experiments. Two-tailed, Mann-Whitney U test; *P < 0.05.