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. 2015 Feb 9;9(1):21–29. doi: 10.4161/19336950.2014.978676

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© 2015 The Author(s) Published with license by Taylor & Francis Group, LLC

© Jaime S Horton, Clay T Wakano, Mark Speck, and Alexander J Stokes

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Overexpression of TPC1, but not TPC2, causes cessation of cellular proliferation and multinucleation. (A) Growth analysis of HEK 293 T-REx cells overexpressing TPC1 or TPC2 induced by tetracycline. Cellular growth based on total protein content measured with the DC protein assay (Bio Rad). Samples were analyzed in triplicate. (B) Representative electron microscope image of wild-type HEK 293 T-REx cells (left) and cells overexpressing TPC1 (right) after 72 h of growth. Scale bar = 5 μm. (C) DNA content of HEK 293 T-REx cells overexpressing TPC1 (top panel) compared to wild-type cells (bottom panel) over 96 h. TPC1 expression was induced by tetracycline treatment, and DNA content assessed by propidium iodide staining by flow cytometry. Increased G2/M accumulation indicated by*. The appearance of >4 N populations are indicated by arrows.