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. 2015 Feb 9;9(1):21–29. doi: 10.4161/19336950.2014.978676

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© 2015 The Author(s) Published with license by Taylor & Francis Group, LLC

© Jaime S Horton, Clay T Wakano, Mark Speck, and Alexander J Stokes

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — TPC1-CIT interaction and localization. (A) Co-immunoprecipitation of TPC1 and CIT-myc. HEK 293 T-REx, TPC1 and TPC2-overexpressing cells were transiently transfected with pCAG vector containing human CIT-myc. Cells were treated with tetracycline, immunoprecipitated with anti-FLAG or anti-myc antibodies and captured onto protein A agarose beads. Samples were resolved by 10% SDS-PAGE and Western blotted for anti-FLAG or anti-myc reactive proteins. TPC1-FLAG, 94 kDa; TPC2-FLAG, 85 kDa; CIT-myc, 231 kDa. (B) Representative fluorescence microscope image of WT HEK 293 T-REx immunostained for endogenous TPC1 (top left panel, red channel), CIT (top right panel, green channel), DNA (bottom left panel, blue channel), and overlay (bottom right panel). Scale bar = 10 μm.