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. 2015 Aug 20;24(10):1649–1659. doi: 10.1002/pro.2760

Figure 3.

Figure 3

GATA1CF but not GATA1NF shows robust binding to DNA. EMSA analysis of (A) GATA1NF (lanes 2–10) or GST-GATA1NF (lanes 13–20) binding to a GATC-containing oligonucleotide at concentrations of 100, 200, 400, 600, 800, 1000, 2000, and 3000 nM at low salt (15 mM NaCl); and GATA1CF binding to (B) GG (lanes 2–10) or GG_M1 (lanes 12–20) at concentrations of 50, 100, 200, 400, 500, 800, 1000, 2000, 5000, and 7500 nM in 15 mM NaCl, or (C) to mPal (lanes 2–10) or mPal_M1 (lanes 12–20) at concentrations of 50, 100, 200, 400, 500, 800, 1000, 2000, 5000, and 7500 nM in 15 mM NaCl.