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. 2015 Aug 20;24(10):1649–1659. doi: 10.1002/pro.2760

Figure 4.

Figure 4

EMSA analysis of GATA1NC binding to double-site DNA. Binding of GATA1NC to (A) mPal (lanes 2–10) or mPal _M1 (lanes 12–20) at 10, 20, 50, 100, 200, 400, 600, 800, and 1000 nM on low salt (15 mM NaCl), (B) mPal (lanes 2–8) or mPal_M1 (lanes 10–15) at concentrations of 0.5, 1, 2, 3, 4, 5, and 6 µM in physiological salt (150 mM NaCl) (C) GG at 20, 50, 100, 200, 400, and 600 nM (lanes 2–7), GG_M1 oligonucleotide at 100, 200, 400, 600, 800, and 1000 nM (lanes 9–14) and to a single site GATA oligonucleotide (1GATA) at 50, 100, 200, 400, and 600 nM (lanes 16–20) in low salt (15 mM NaCl), (D) GG (lanes 2–8) and GG_M1 (lanes 9–16) at 0.5, 1, 2, 3, 4, 5, and 6 µM in physiological salt (150 mM NaCl). Schematics of complexes (indicated with numbered arrows) show the GATA1NF in white and GATA1CF in black. Note that the change in intensity throughout the final lane of panel C is an artefact from phosphorimaging.