Table 1.
Differentially Sialylated Proteins Identified by Mass Spectrometry in Absence or Presence of TGFBR2 Using HCT116-TGFBR2 #5 in at Least Two Out of Three Replicates
| Accessiona | Description | Mass (Da) | MASCOT score | Signif. peptidesb | Coverage (%)c |
|---|---|---|---|---|---|
| TGFBR2-deficient identified proteins (-Dox) | |||||
| DDX3X | ATP-dependent RNA helicase DDX3X | 73597 | 82/42 | 1/0 | 4/3 |
| DKK1 | Dickkopf-related protein 1 | 29793 | 52/36 | 1/1 | 3/3 |
| H31 | Histone H3.1 | 15509 | 97/136 | 1/1 | 34/38 |
| ITB3 | Integrin beta-3 | 90194 | 34/34 | 1/1 | 1/1 |
| NUCB1 | Nucleobindin-1 | 53846 | 79/57 | 1/1 | 11/4 |
| SNX14 | Sorting nexin-14 | 111081 | 30/30 | 0/0 | 1/1 |
| TBB2A | Tubulin beta-2A chain | 50274 | 62/80 | 1/2 | 6/6 |
| TGFBR2-proficient identified proteins (+Dox) | |||||
| 1433G | 14-3-3 protein gamma | 28456 | 201/182/93 | 2/3/1 | 21/22/11 |
| 1A69 | HLA class I histocompatibility antigen, A-69 alpha chain | 41236 | 202/147 | 3/2 | 22/20 |
| 1B35 | HLA class I histocompatibility antigen, B-35 alpha chain | 40715 | 182/171 | 2/3 | 20/17 |
| AATC | Aspartate aminotransferase, cytoplasmic | 46447 | 64/45 | 1/0 | 5/4 |
| ACTN4 | Alpha-actinin-4 | 105245 | 70/72 | 0/1 | 2/3 |
| ARF5 | ADP-ribosylation factor 5 | 20631 | 78/78 | 1/0 | 17/17 |
| AT1A1 | Sodium/potassium-transporting ATPase subunit alpha-1 | 114135 | 138/189/130 | 2/3/2 | 5/5/4 |
| C1QBP | Complement component 1 Q subcomponent-binding protein, mitochondrial | 31742 | 30/48 | 1/1 | 11/11 |
| CAP1 | Adenylyl cyclase-associated protein 1 | 52325 | 33/43 | 1/0 | 4/6 |
| CD109 | CD109 antigen | 162500 | 53/81/51 | 0/1/1 | 2/3/2 |
| CLIC1 | Chloride intracellular channel protein 1 | 27248 | 90/64/63 | 1/1/1 | 17/10/10 |
| DHX9 | ATP-dependent RNA helicase A | 142181 | 130/123 | 3/1 | 4/4 |
| EFNB2 | Ephrin-B2 | 37356 | 37/32 | 1/1 | 4/4 |
| EIF3H | Eukaryotic translation initiation factor 3 subunit H | 40076 | 37/36 | 1/1 | 5/5 |
| ELAV1 | ELAV-like protein 1 | 36240 | 46/68 | 0/1 | 6/6 |
| EPHA2 | Ephrin type-A receptor 2 | 109679 | 45/35 | 1/1 | 1/1 |
| FKBP4 | Peptidyl-prolyl cis-trans isomerase FKBP4 | 52057 | 69/41 | 0/0 | 7/4 |
| FLNB | Filamin-B | 280157 | 75/121 | 1/2 | 1/1 |
| FSCN1 | Fascin | 55123 | 65/138/75 | 1/2/0 | 5/10/6 |
| G6PI | Glucose-6-phosphate isomerase | 63335 | 61/34 | 2/1 | 4/1 |
| GBB1 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | 38151 | 42/92 | 1/1 | 3/7 |
| GBG5 | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5 | 7428 | 58/52 | 0/0 | 24/24 |
| HNRPC | Heterogeneous nuclear ribonucleoproteins C1/C2 | 33707 | 86/77 | 2/1 | 12/12 |
| HNRPL | Heterogeneous nuclear ribonucleoprotein L | 64720 | 31/79 | 1/1 | 5/8 |
| IF2A | Eukaryotic translation initiation factor 2 subunit 1 | 36374 | 38/44 | 1/1 | 4/4 |
| IMDH2 | Inosine-5∼-monophosphate dehydrogenase 2 | 56226 | 64/75/36 | 1/1/1 | 5/5/2 |
| IMPA3 | Inositol monophosphatase 3 | 38828 | 40/33 | 1/1 | 3/3 |
| ITA5 | Integrin alpha-5 | 115605 | 73/96/127 | 2/2/2 | 2/4/4 |
| ITB5 | Integrin beta-5 | 91303 | 52/81/54 | 1/2/1 | 2/3/3 |
| K2C8 | Keratin, type II cytoskeletal 8 | 53671 | 145/146 | 1/2 | 7/8 |
| LAT1 | Large neutral amino acids transporter small subunit 1 | 55659 | 51/62 | 0/1 | 7/7 |
| LEG1 | Galectin-1 | 15048 | 92/45/48 | 0/0/0 | 33/15/13 |
| LPPRC | Leucine-rich PPR motif-containing protein, mitochondrial | 159003 | 31/74 | 0/1 | 1/2 |
| NDKB | Nucleoside diphosphate kinase B | 17401 | 109/103 | 3/1 | 23/22 |
| NICA | Nicastrin | 79103 | 108/88 | 0/0 | 5/5 |
| NONO | Non-POU domain-containing octamer-binding protein | 54311 | 40/71/54 | 1/2/1 | 2/7/7 |
| P5CS | Delta-1-pyrroline-5-carboxylate synthase | 87989 | 67/31 | 1/0 | 3/2 |
| PCBP1 | Poly(rC)-binding protein 1 | 37987 | 122/80/146 | 3/2/2 | 10/6/12 |
| PDIA1 | Protein disulfide-isomerase | 57480 | 141/50/52 | 3/0/1 | 13/6/2 |
| PDIA4 | Protein disulfide-isomerase A4 | 73229 | 85/34/37 | 2/1/1 | 4/2/2 |
| PLIN3 | Perilipin-3 | 47217 | 68/63 | 1/1 | 5/5 |
| PORIM | Porimin | 21575 | 51/51 | 0/0 | 7/7 |
| PPIB | Peptidyl-prolyl cis-trans isomerase B | 23785 | 153/181/82 | 3/2/2 | 24/19/13 |
| PRDX5 | Peroxiredoxin-5, mitochondrial | 22301 | 125/32/53 | 1/1/0 | 25/4/12 |
| PRDX6 | Peroxiredoxin-6 | 25133 | 88/58/35 | 1/1/1 | 13/10/4 |
| PSB5 | Proteasome subunit beta type-5 | 28633 | 36/57 | 1/1 | 4/6 |
| PVRL3 | Poliovirus receptor-related protein 3 (nectin-3) | 61477 | 52/35 | 1/1 | 2/2 |
| RAB1A | Ras-related protein Rab-1A | 22891 | 60/82 | 1/2 | 8/11 |
| RL10A | 60S ribosomal protein L10a | 24987 | 44/37 | 1/1 | 4/4 |
| RL30 | 60S ribosomal protein L30 | 12947 | 33/69 | 1/1 | 10/17 |
| RL34 | 60S ribosomal protein L34 | 13513 | 59/56 | 1/1 | 15/15 |
| RL6 | 60S ribosomal protein L6 | 32765 | 55/56/52 | 2/1/1 | 5/5/5 |
| RL7A | 60S ribosomal protein L7a | 30148 | 39/99 | 1/2 | 4/12 |
| RS11 | 40S ribosomal protein S11 | 18590 | 46/81 | 1/2 | 10/15 |
| RS26 | 40S ribosomal protein S26 | 13292 | 49/77 | 0/1 | 21/21 |
| RS27L | 40S ribosomal protein S27-like | 9813 | 34/33 | 1/1 | 10/10 |
| SCRB2 | Lysosome membrane protein 2 | 54712 | 105/68 | 1/1 | 8/4 |
| STIP1 | Stress-induced-phosphoprotein 1 | 63227 | 39/44 | 1/0 | 2/3 |
| SYNC | Asparagine–tRNA ligase, cytoplasmic | 63758 | 55/34 | 1/1 | 3/2 |
| SYNE1 | Nesprin-1 | 1017127 | 75/51/55 | 1/1/1 | 0.3/0.2/0.2 |
| SYVC | Valine–tRNA ligase | 141642 | 36/40 | 1/1 | 1/2 |
| TAGL2 | Transgelin-2 | 22548 | 62/35 | 1/1 | 15/6 |
| TM87A | Transmembrane protein 87A | 63788 | 31/59 | 0/1 | 2/3 |
| TMED4 | Transmembrane emp24 domain-containing protein 4 | 26097 | 50/55 | 0/0 | 8/9 |
| TMED9 | Transmembrane emp24 domain-containing protein 9 | 27374 | 53/63 | 1/2 | 7/7 |
| TPM3L | Putative tropomyosin alpha-3 chain-like protein | 26595 | 75/113 | 1/1 | 10/10 |
| TRFM | Melanotransferrin | 81760 | 89/65 | 2/1 | 4/3 |
| UBE2N | Ubiquitin-conjugating enzyme E2 N | 17184 | 33/49 | 1/1 | 7/7 |
| VDAC1 | Voltage-dependent anion-selective channel protein 1 | 30868 | 46/77/58 | 0/1/2 | 6/9/6 |
| XPO2 | Exportin-2 | 111145 | 57/68/43 | 1/2/1 | 3/2/2 |
Slashes correspond to individual experiments of triplicates.
a
Proteins identified by the SwissProt 2013_02 database with taxonomy set to human.
b
Significant peptides.
c
Sequence coverage includes all identified peptides.